Glycolysis
• The Glycolytic pathway describes the oxidation of glucose to pyruvate with the generation of ATP and NADH
• It is also called as the Embden-Meyerhof Pathway
• Glycolysis is a universal pathway; present in all organisms: from yeast to mammals.
• In eukaryotes, glycolysis takes place in the cytosol
• Glycolysis is anaerobic; it does not require oxygen
• In the presence of O2, pyruvate is further oxidized to CO2. In the absence of O2, pyruvate can be fermented to lactate or ethanol.
• Net Reaction: Glucose + 2NAD+ + 2 Pi + 2 ADP = 2 pyruvate + 2 ATP + 2 NADH + 2 H2O
The 3 stages of Glycolysis
• Stage 1 is the investment stage. 2 mols of ATP are consumed for each mol of glucose
• Glucose is converted to fructose-1,6-bisphosphate.
• Glucose is trapped inside the cell and at the same time converted to an unstable form that can be readily cleaved into 3-carbon units.
• In stage 2 fructose-1,6-bisphosphate is cleaved into 2 3- carbon units of glycerladehyde-3-phosphate.
• Stage 3 is the harvesting stage. 4 mols of ATP and 2 mols of NADH are gained from each initial mol of glucose. This ATP is a result of substrate-level phosphorylation
• Glyceraldehyde-3-phosphate is oxidized to pyruvate
Step-wise reactions of glycolysis
• Reaction 1: Phosphorylation of glucose to glucose-6 phosphate.
• This reaction requires energy and so it is coupled to the
hydrolysis of ATP to ADP and Pi.
• Enzyme: hexokinase. It has a low Km for glucose; thus, once glucose enters the cell, it gets phosphorylated.
• This step is irreversible. So the glucose gets trapped inside the cell. (Glucose transporters transport only free glucose, not phosphorylated glucose)
• Reaction 2: Isomerization of glucose-6-phosphate to fructose 6- phosphate. The aldose sugar is converted into the keto isoform.
• Enzyme: phosphoglucomutase.
• This is a reversible reaction. The fructose-6-phosphate is quickly consumed and the forward reaction is favored.
Step-wise reactions of glycolysis (continued)
• Reaction 3: is another kinase reaction. Phosphorylation of the hydroxyl group on C1 forming fructose-1,6- bisphosphate.
• Enzyme: phosphofructokinase. This allosteric enzyme regulates the pace of glycolysis.
• Reaction is coupled to the hydrolysis of an ATP to ADP and Pi.
• This is the second irreversible reaction of the glycolytic pathway.
• Reaction 4: fructose-1,6-bisphosphate is split into 2 3-carbon molecules, one aldehyde and one ketone: dihyroxyacetone phosphate (DHAP) and glyceraldehyde 3-phosphate (GAP).
• The enzyme is aldolase.
• Reaction 5: DHAP and GAP are isomers of each other and can readily inter-convert by the action of the enzyme triose-phosphate isomerase.
• GAP is a substrate for the next step in glycolysis so all of the DHAP is eventually depleted. So, 2 molecules of GAP are formed from each molecule of glucose
Step-wise reactions of glycolysis (continued)
• Upto this step, 2 molecules of ATP were required for each molecule of glucose being oxidized
• The remaining steps release enough energy to shift the balance sheet to the positive side. This part of the glycolytic pathway is called as the payoff or harvest stage.
• Since there are 2 GAP molecules generated from each glucose, each of the remaining reactions occur twice for each glucose molecule being oxidized.
• Reaction 6: GAP is dehydrogenated by the enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH). In the process, NAD+ is reduced to NADH + H+ from NAD. Oxidation is coupled to the phosphorylation of the C1 carbon. The product is 1,3-bisphosphoglycerate.
Step-wise reactions of glycolysis (continued)
• Reaction 7: BPG has a mixed anhydride, a high energy bond, at C1. This high energy bond is hydrolyzed to a carboxylic acid and the energy released is used to generate ATP from ADP. Product: 3-phosphoglycerate. Enzyme: phosphoglycerate kinase.
• Reaction 8: The phosphate shifts from C3 to C2 to form 2- phosphoglycerate. Enzyme:phosphoglycerate mutase.
• Reaction 9: Dehydration catalyzed by enolase (a lyase). A water molecule is removed to form phosphoenolpyruvate which has a double bond between C2 and C3.
• Reaction 10: Enolphosphate is a high energy bond. It is hydrolyzed to form the enolic form of pyruvate with the synthesis of ATP. The irreversible reaction is catalyzed by the enzyme pyruvate kinase. Enol pyruvate quickly changes to keto pyruvate which is far more stable.
Glycolysis: Energy balance sheet
• Hexokinase: - 1 A TP
• Phosphofructokinase: -1 A TP
• GAPDH: +2 NADH
• Phsophoglycerate kinase:
• Pyruvate kinase:
Total/ molecule of glucose:
+2 A TP +2 A TP
+2 ATP, +2 NADH
Fate of Pyruvate
• NADH is formed from NAD+ during glycolysis.
• The redox balance of the cell has to be maintained for further cycles of glycolysis to continue.
• NAD+ can be regenerated by one of the following reactions /pathways:
• Pyruvate is converted to lactate
• Pyruvate is converted to ethanol
• In the presence of O2, NAD+ is regenerated by ETC. Pyruvate is converted to acetyl CoA which enters TCA cycle and gets completely oxidized to CO2.
Lactate Fermentation
• Formation of lactate catalyzed by lactate dehydrogenase:
CH3-CO-COOH + NADH + H+ CH3-CHOH-COOH + NAD+
• In highly active muscle, there is anaerobic glycolysis because the supply of O2 cannot keep up with the demand for ATP.
• Lactate builds up causing a drop in pH which inactivates glycolytic enzymes. End result is energy deprivation and cell death; the symptoms being pain and fatigue of the muscle.
• Lactate is transported to the liver where it can be reconverted to pyruvate by the LDH reverse reaction
Ethanol fermentation
• Formation of ethanol catalyzed by 2 enzymes
• Pyruvate decarboxylase catalyzes the first irreversible reaction to form acetaldehyde:
CH3-CO-COOHCH3-CHO + CO2
• Acetaldehyde is reduced by alcohol dehydogenase is a
reversible reaction:
CH3-CHO + NADH + H+ CH3CH2OH + NAD+
• Ethanol fermentation is used during wine-making
Entry of other sugars into glycolysis
• Fructose is phosphorylated by fructokinase (liver) or hexokinase (adipose) on the 1 or 6 positions resp.
• Fructose-6-phosphate is an intermediate of glycolysis.
• Fructose-1-phosphate is acted upon by an aldolase-like enz that gives DHAP and glyceraldehyde.
• DHAP is a glycolysis intermediate and glyceraldehyde can be phosphorylated to glyceraldehyde-3-P.
• Glycerol is phosphorylated to G-3-P which is then converted to glyceraldehyde 3 phosphate.
• Galactose has a slightly complicated multi-step pathway for conversion to glucose-1-phosphate.
• galgal-1-PUDP-galUDP-glcglc-1-P.
• If this pathway is disrupted because of defect in one or more enz involved in the conversion of gal to glc-1-P, then galactose accumulates in the blood and the subject suffers from galactosemia which is a genetic disorder, an inborn error of metabolism.
Regulation of Glycolysis
Enzyme
Hexokinase Phosphofructokinase
Pyruvte kinase
Enzyme
Hexokinase Phosphofructokinase Pyruvate kinase
Activator
AMP/ADP AMP/ADP ,
Fructose-2,6-bisphosphate AMP/ADP
Fructose-1,6-bisphosphate
Inhibitor
Glucose-6-phosphate ATP, Citrate
ATP, Acetyl CoA, Alanine
Gluconeogenesis
• Gluconeogenesis is the synthesis of glucose from non- carbohydrate precursors including pyruvate, lactate, glycerol and aminoacids
• In animals the gluconeogenesis pathway is, for the most part, the reverse of glycolysis. There are substitute or bypass reactions for the irreversible steps of glycolysis.
• Glycerol enters reverse glycolysis as DHAP by the action of glycerol kinase followed by dehydrogenase
• Lacate is converted to pyruvate by LDH. Aminoacids are converted to either pyruvate or oxaloacetate prior to gluconeogenesis.
Bypass for Puruvate Kinase
• Three steps of glycolysis are irreversible and therefore need bypass reactions for gluconeogenesis.
• Pyruvate to PEP: Pyruvate synthesized by glycolysis or from aa is in the mitochondria. Here, pyruvate is first converted to oxaloacetate by the enzyme pyruvate carboxylase. One carbon is supplied by CO2 to form the 4-C oxaloacetate. The reaction is coupled to ATP hydrolysis making this a ligation reaction.
• Oxaloacetate is shuttled out to the cytoplasm where the glycolytic enzymes are located. Oxaloacetate is converted to PEP by the enzyme PEP carboxykinase. CO2 is removed and energy in the form of GTP is utilized.
• Two high energy molecules with a total free energy change of 62 kJ/mol are used up for the formation of PEP. This is consistent with the free energy change for hydrolysis of the enoyl phosphate bond.
• Net Reaction for gluconeogenesis:
• 2pyruvate+ 4ATP+2GTP+2NADH+2H+ +6H2Oglucose+2NAD+ +4ADP+2GDP+6Pi.
• (Net reaction for glycolysis is: Glucose + 2NAD+ + 2 ADP + 2 Pi2 pyruvate + 2 ATP + 2 NADH + 2 H2O)
Cori Cycle
• Lactate is formed in the active muscle to regenerate NAD+ from NADH so that glycolysis can continue.
• The muscle cannot spare NAD+ for re-conversion of lactate back to pyruvate.
• Thus, lactate is transported to the liver, where, in the presence of oxygen, it undergoes gluconeogenesis to form glucose.
• The glucose is supplied by the liver to various tissues including muscle.
• This inter-organ cooperation during high muscular activity is called as the Cori cycle.
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